16S rRNA sequences used to identification of bacterial DNA isolated from various materials
The aim of this study was the genetic analysis of two different material. The whole DNA was isolated from the air filter, located in Integrated Environmental Monitoring Station on Swiety Krzyz in the Swietokrzyskie Mountains. The second sample of whole DNA was obtained from sludge. Total DNA was extracted by two method – temperature
isolation and column isolation kit. Genetic analysis revealed presence only Eubacteria. The confirmation of the presence of Archaeacan be possible during further optimization of the used techniques. Presented results confirmed that air filters and sludge are the useful materials to isolate bacterial DNA to identification and to look for microorganisms which could possess some unique factors for adapt to environment. Air filters or sludge can be use
to metagenomic analysis of microorganisms present in different environment, especially to the identification of uncultured bacteria from environment.